This book has sold over 10,000 copies since its publication in 1986, and remains one of the most popular titles in the Practical Approach Series. This new, thoroughly updated edition details the recent progress made in this field. There have been significant advances in areas such as serum-free media, scale-up, and flow cytometry. As these techniques have developed as tools for cell biologists, their availability to the non-specialist has also increased dramatically. Use of the tetrazolium salt MTT as a colorimetric indicator of viability has made a considerable impact on cytotoxicity assay, and DNA fingerprinting has revolutionized the identification of individual cell strains. These and other developments in the techniques described have made this new edition essential. This cohesive volume presents techniques in a readily accessible form, and provides detailed protocols throughout. This volume will be of use to researchers involved in both biological research and the commercial exploitation of animal cell culture.