Controlling enantioselective separations of chiral compounds is one of the important challenges in chemistry. The enantioselectivity of chiral chemicals usually requires the use of chiral media such as chiral catalysts for synthesis and chiral stationary phases for chromatographic separations. This study has been devoted to the preparation, characterization and applications of chiral nanosurfaces in enantioselective chemical processes. The first part of the book focuses on the development of chiral mesoporous silica based on the imprinting approach using chiral organic templates such as fatty acid micelles and collagen protein. The second part focuses on the preparation and investigation of templated chiral alumina nanosurfaces using atomic layer deposition (ALD) process on chiral self-assembled monolayers (SAMs) surfaces.