This is the 12th annual report of the results of the Federal Transit Administration’s (FTA) Drug and Alcohol Testing Program. This report summarizes the reporting requirements for calendar year 2006, the requirements of the overall drug and alcohol testing program (49 CFR Part 40 and 49 CFR Part 655), the results from the data reported for 2006, and the random drug and alcohol violation rates (the percentage of persons selected for a random test who produced a positive specimen or refused to take the test) for calendar years 1995 through 2006.This report provides a brief overview of the drug and alcohol testing requirements for both safety-sensitive employers and employees. The official random rates for 2006 are provided, as well as the official random rate trends over the last 12 years. The results of alcohol and drug tests are provided by test type, employee category, and region. The positive testing rates for drugs andalcohol are also provided for each test type, employee category, and region.