This book gives a broad account of enzymology, putting current knowledge into perspective. The chapters follow a progression from the properties of isolated enzymes to the behaviour of enzymes in increasingly complex systems, leading up to the cell. Included is a discussion on the importance of enzymes in medicine and industry. The book discusses the behaviour of isolated enzymes, dealing in turn with isolation methods, structural characterization, kinetics, catalytic action and control of activity, immobilization methods and various applications of enzymes. The methods for isolation and characterization of enzymes are now well-established procedures, so the rate at which three-dimensional structures and mechanisms are being determined is increasing dramatically. Ultimately it is necessary to know the behaviour of enzymes in living cells. This involves in part a synthesis of the information obtained from the study of isolated enzymes, but it also requires detailed knowledge of the molecular morphology of the cell, which in turn requires methods for making measurements on intact cells. The study and applications of enzymes have assumed increasing importance both in medicine and in industry and a discussion of these aspects is given prime importance. This book will be of use to students of biotechnology engineering and science, and research scholars. Apart from updating the complete text wherever required new material has been added., including Mechanism of Enzyme Activities in Organic Solvents and Immobilization Kinetics .