Oxidants and Antioxidants Ultrastructure and Molecular Biology Protocols

Auteur: D. Armstrong
Taal: Engels
Oxidants and Antioxidants
Auteur: D. Armstrong
Uitgever: Humana Press Inc.
  • Engels
  • Hardcover
  • 9780896038516
  • Druk: 2002 ed.
  • juli 2002
  • 356 pagina's
Alle productspecificaties

Samenvatting

In our first protocols book, Free Radical and Antioxidant Protocols (1), r- erence to in vivo, ex vivo, or in situ techniques were few compared to classical biochemical assays and only 6 of the 40 chapters were concerned with these applications. In our second book, Oxidative Stress Biomarkers and Antioxidant Protocols (2), which is being published concurrently with this third volume, Oxidants and Antioxidants: Ultrastructure and Molecular Biology Protocols, the number of such chapters has increased. The literature dealing with histoche- cal/cytochemical and immunohistochemical techniques and staining to identify cellular/subcellular sites of oxidative stress has expanded rapidly, as has the molecular biology methodology used to analyze free radical and antioxidant (AOX) reactions, as well as the monitoring of living tissue. A two-way search was performed for each technique listed in Table 1, coupled with oxidative stress using the PUBMED search engine from the National Library of Medicine at NIH. Most of the techniques involved in m- suring oxidative stress employ molecular biology or ultrastructural approaches. Of these techniques, histology, polymerase chain reaction, and Western blotting are the most widely used. Several forms of therapy are now available for patients with increased oxidative stress. In addition to standard antioxidant therapy supplementation in vivo and in vitro, photodynamic therapy (PDT) employs excitation of a photon-emitting compound delivered systemically for free radical-mediated necrosis of affected tissues, and stem cells are also being used to induce signaling events or replace antioxidant enzymes.

Recensie(s)

...this volume is unique on its focus on protocols for identification and localization of oxidants, using molecular biology and other types of applications both in-vitro and in-vivo. This book is recommended for all biomedical research libraries and researchers at all levels who are interested in oxidative stress measurement. - E-STREAMS

Productspecificaties

Inhoud

Taal
Engels
Bindwijze
Hardcover
Druk
2002 ed.
Verschijningsdatum
2002-07-01
Aantal pagina's
356 pagina's
Illustraties
Nee

Betrokkenen

Auteur
D. Armstrong
Redacteur
Donald Armstrong
Uitgever
Humana Press Inc.

EAN

EAN
9780896038516

Overige kenmerken

Extra groot lettertype
Nee
NUR code
910
Oorspronkelijke releasedatum
2002-07-01
Subtitel
Ultrastructure and Molecular Biology Protocols
Thema Subject Code
PSB

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