RNAi and microRNA-Mediated Gene Regulation in Stem Cells Methods, Protocols, and Applications

RNAi and microRNA-Mediated Gene Regulation in Stem Cells
Auteur: Zhang
Uitgever: Humana Press Inc.
  • Engels
  • Hardcover
  • 9781607617686
  • Druk: 2010
  • augustus 2010
  • 261 pagina's
Alle productspecificaties

Samenvatting

Recent stem cell research has revealed that miRNA and RNAi-mediated gene regulation is one of the vital determinates controlling the state of cell differentiation, with the small RNAs serving as key elements involved in regulatory network control of pluripotent cell fate determination. In RNAi and microRNA-Mediated Gene Regulation in Stem Cells: Methods, Protocols, and Applications, expert authors from laboratories across the globe contribute an accessible compendium of up-to-date, proven methods focused on the study of the titular topic. Divided into three sections, the book first gives a brief introduction to RNAi and miRNAs in stem cells, with a focus on the current status of research and future perspectives, then it continues with detailed methods and protocols for RNAi screening, transfection, and the knockdown of specific genes and pathways in several animal species, including humans and mice, concluding with a section on recently developed methods for identification of miRNAs, including a general protocol for preparation and analysis of miRNA libraries for deep sequencing, knock down of a specific gene using miRNAbased shRNA, and miRNA expression analysis using qRT-PCR. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, RNAi and microRNA-Mediated Gene Regulation in Stem Cells: Methods, Protocols, and Applications serves as a valuable resource for scientists and aspiring graduate students interested in the intersection of RNAi, miRNA, and stem cell molecular biology and the exciting areas of medicine, including regenerative medicine, aging, cancer, and neurological disorders, that can be advanced through this expanding area of research.

Productspecificaties

Inhoud

Taal
Engels
Bindwijze
Hardcover
Druk
2010
Verschijningsdatum
2010-07-01
Aantal pagina's
261 pagina's
Illustraties
Nee

Betrokkenen

Auteur
Zhang
Redacteur
Baohong Zhang
Uitgever
Humana Press Inc.

EAN

EAN
9781607617686

Overige kenmerken

Extra groot lettertype
Nee
Oorspronkelijke releasedatum
2010-08-16
Subtitel
Methods, Protocols, and Applications
Thema Subject Code
PSF

Je vindt dit artikel in

Categorieën
Taal
Engels
Studieboek of algemeen
Algemene boeken
Onderwerp
Biologie, Levenswetenschappen, Geneeskunde
Boek, ebook of luisterboek?
Boek
Nog geen reviews
Bindwijze: Hardcover
142 70
1 - 2 weken Tooltip
Verkoop door partner van bol.com Books2Anywhere
Andere verkopers (1)
Snelste levering
Uiterlijk 14 december in huis
Snelste levering
  • Gratis verzending
  • 30 dagen bedenktijd en gratis retourneren