In Vitro Maturation of Bovine Oocytes Effect of cumulus cells and ovum retrieval methods

Twenty first century is heading towards a revolution in the application of biotechnological procedures such as nuclear transfer cloning, sperm and embryo sexing, production of transgenic animals, stem cell research etc. in animals. In addition, the conservation of endangered species and rare livestock breeds are also possible by IVM and IVF techniques. The successful application of these techniques is critically dependent on the expertise in the basic reproduction techniques such as in vitro maturation of oocytes, IVF and in vitro culture of embryos. Improvement in the efficiency of in vitro maturation procedure could improve the quality and quantity of IVF/ IVP embryos. Lot of factors starting right from medium to quality of oocytes, decide the efficiency of IVM. It was in this context this study was undertaken in cattle.