Methods in Molecular Biology- Plant-Nematode Interactions
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- Engels
- Hardcover
- 9781071636374
- 02 maart 2024
- 384 pagina's
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This volume explores the latest advancements and techniques used by researchers to work with plant parasitic nematodes (PPNs) and to study their interactions with crop plants.
This volume explores the latest advancements and techniques used by researchers to work with plant parasitic nematodes (PPNs) and to study their interactions with crop plants. The chapters in this book cover topics such as ways to obtain samples from soil and different plant tissues; rearing nematodes in greenhouse setting; methods used to axenically rear nematodes in vitro; Denaturing Gradient Gel Electrophoresis (DGGE) separation; methods for ROS detection and hydrogen peroxide quantification; methods to elicit plant resistance against nematodes; methods for the detection of Pathogenesis Related (PR) genes that are the executioners of immunity in plant-RKN interactions; and laboratory procedures that test nematodes on their isolation, count, culture, identification, pathogenicity, virulence, and environmental tolerance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Plant-Nematode Interactions is a valuable tool for any researcher interested in learning more about this interesting and developing field.
This volume explores the latest advancements and techniques used by researchers to work with plant parasitic nematodes (PPNs) and to study their interactions with crop plants. The chapters in this book cover topics such as ways to obtain samples from soil and different plant tissues; rearing nematodes in greenhouse setting; methods used to axenically rear nematodes in vitro; Denaturing Gradient Gel Electrophoresis (DGGE) separation; methods for ROS detection and hydrogen peroxide quantification; methods to elicit plant resistance against nematodes; methods for the detection of Pathogenesis Related (PR) genes that are the executioners of immunity in plant-RKN interactions; and laboratory procedures that test nematodes on their isolation, count, culture, identification, pathogenicity, virulence, and environmental tolerance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Plant-Nematode Interactions is a valuable tool for any researcher interested in learning more about this interesting and developing field.
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- Taal
- en
- Bindwijze
- Hardcover
- Oorspronkelijke releasedatum
- 02 maart 2024
- Aantal pagina's
- 384
Betrokkenen
- Hoofdredacteur
- Sergio Molinari
- Hoofduitgeverij
- Springer-Verlag New York Inc.
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- Editie
- 24001
- Product breedte
- 178 mm
- Product lengte
- 254 mm
- Verpakking breedte
- 178 mm
- Verpakking hoogte
- 27 mm
- Verpakking lengte
- 254 mm
- Verpakkingsgewicht
- 947 g
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- EAN
- 9781071636374
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