High-Level Gentamicin Resistant Environmental Gram-Negative Bacteria Dissemination of Antibiotic Resistance Genes Through Mobile Molecular Elements

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  • 9783659140082
  • 17 juli 2012
  • 168 pagina's
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Salmonella enterica subsp. arizonae and other bacteria from turtle farming environments were found to be resistant to high concentrations of gentamicin and to other antibiotics. This study identified the genes contributing to the resistance phenotypes observed in diverse bacteria, and explored the molecular elements involved in their dissemination. Cloning and sequencing of the gentamicin resistance genes revealed the presence of aminoglycoside acetyltransferase genes aac(3)IIa and aac(3)VIa. The antibiotic resistance determinants were transferred by conjugation and transformation from resistant bacteria to naive Escherichia coli cells. Pulsed-field gel electrophoresis and RFLP analysis confirmed the genes were carried on mobile genetic elements on different R-plasmids; aac(3)VIa was present as a gene cassette of a class-1 integron, and aac(3)IIa may be encoded as part of a complex class-1 integron. The presence of these genes on mobile molecular elements explains their dissemination among diverse environmental bacteria. This study shows the impact of antibiotic selective pressure and warns against non-clinical use of antibiotics utilized to treat infectious disease in humans."

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Oorspronkelijke releasedatum
17 juli 2012
Aantal pagina's
168

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Hoofdauteur
Maria Alejandra Diaz

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Product breedte
152 mm
Product hoogte
10 mm
Product lengte
229 mm
Verpakking breedte
152 mm
Verpakking hoogte
10 mm
Verpakking lengte
229 mm
Verpakkingsgewicht
254 g

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EAN
9783659140082

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