Advanced Technologies for Protein Complex Production and Characterization 2016

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  • Engels
  • Hardcover
  • 9783319272146
  • 20 mei 2016
  • 382 pagina's
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Samenvatting

This book presents advanced expression technologies for the production of protein complexes. Since complexes lie at the heart of modern biology, the expression, purification, and characterization of large amounts of high-quality protein complexes is crucial for the fields of biomedicine, biotechnology, and structural biology. From co-expression in E. coli, yeast, mammalian and insect cells to complex reconstitution from individual subunits, this book offers useful insights and guidance for successful protein expressionists.

Across several sections readers will discover existing opportunities for the production of protein complexes in bacterial systems (including membrane proteins and cell-free co-expression), methylotrophic and non-methylotrophic yeasts, protozoa (Leishmania terantolae and Dictyostelium discoideum), baculovirus-infected insect cells, mammalian cells, plants and algae. Complex reconstitution from individually purified subunits or subcomplexes is discussed as a complementary strategy. A last section introduces briefly some of the biophysical and structural characterization techniques for macromolecular complexes using state-of-the-art solution scattering and nuclear magnetic resonance.

This work is a guided tour over some of the most powerful and successful protein expression technologies, with a focus on co-expression and high-throughput applications. It is addressed to everyone interested in the production and characterization of macromolecular complexes, from university students who want an accessible description of the major co-expression systems to researchers in biomedicine and the life sciences seeking for an up-to-date survey of available technologies.

Productspecificaties

Inhoud

Taal
en
Bindwijze
Hardcover
Oorspronkelijke releasedatum
20 mei 2016
Aantal pagina's
382
Illustraties
Nee

Betrokkenen

Hoofdredacteur
M. Cristina Vega

Overige kenmerken

Editie
1st ed. 2016
Extra groot lettertype
Nee
Product breedte
187 mm
Product hoogte
27 mm
Product lengte
264 mm
Studieboek
Ja
Verpakking breedte
178 mm
Verpakking hoogte
254 mm
Verpakking lengte
254 mm
Verpakkingsgewicht
928 g

EAN

EAN
9783319272146

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