Protein Sequencing and Identification Using Tandem Mass Spectrometry
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Auteur:
Michael Kinter
Nicholas E. Sherman
- Engels
- Hardcover
- 9780471322498
- 09 oktober 2000
- 320 pagina's
Samenvatting
This volume describes the complete process of analyzing protein sequences by mass spectrometry including sample preparation, analysis, and data interpretation at a practical level. It provides sufficient experimental detail to carry out the experiments and consistently achieve good results.
How to design, execute, and interpret experiments for protein sequencing using mass spectrometry
The rapid expansion of searchable protein and DNA databases in recent years has triggered an explosive growth in the application of mass spectrometry to protein sequencing. This timely and authoritative book provides professionals and scientists in biotechnology research with complete coverage of procedures for analyzing protein sequences by mass spectrometry, including step-by-step guidelines for sample preparation, analysis, and data interpretation.
Michael Kinter and Nicholas Sherman present their own high-quality, laboratory-tested protocols for the analysis of a wide variety of samples, demonstrating how to carry out specific experiments and obtain fast, reliable results with a 99% success rate. Readers will get sufficient experimental detail to apply in their own laboratories, learn about the proper selection and operation of instruments, and gain essential insight into the fundamental principles of mass spectrometry and protein sequencing. Coverage includes:
* Peptide fragmentation and interpretation of product ion spectra
* Basic polyacrylamide gel electrophoresis
* Preparation of protein digests for sequencing experiments
* Mass spectrometric analysis using capillary liquid chromatography
* Techniques for protein identification by database searches
* Characterization of modified peptides using tandem mass spectrometry
And much more
How to design, execute, and interpret experiments for protein sequencing using mass spectrometry
The rapid expansion of searchable protein and DNA databases in recent years has triggered an explosive growth in the application of mass spectrometry to protein sequencing. This timely and authoritative book provides professionals and scientists in biotechnology research with complete coverage of procedures for analyzing protein sequences by mass spectrometry, including step-by-step guidelines for sample preparation, analysis, and data interpretation.
Michael Kinter and Nicholas Sherman present their own high-quality, laboratory-tested protocols for the analysis of a wide variety of samples, demonstrating how to carry out specific experiments and obtain fast, reliable results with a 99% success rate. Readers will get sufficient experimental detail to apply in their own laboratories, learn about the proper selection and operation of instruments, and gain essential insight into the fundamental principles of mass spectrometry and protein sequencing. Coverage includes:
* Peptide fragmentation and interpretation of product ion spectra
* Basic polyacrylamide gel electrophoresis
* Preparation of protein digests for sequencing experiments
* Mass spectrometric analysis using capillary liquid chromatography
* Techniques for protein identification by database searches
* Characterization of modified peptides using tandem mass spectrometry
And much more
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Inhoud
- Taal
- en
- Bindwijze
- Hardcover
- Oorspronkelijke releasedatum
- 09 oktober 2000
- Aantal pagina's
- 320
- Illustraties
- Nee
Betrokkenen
- Hoofdauteur
- Michael Kinter
- Tweede Auteur
- Nicholas E. Sherman
- Co Auteur
- Kinter
- Hoofduitgeverij
- Wiley-Interscience
Overige kenmerken
- Editie
- 5
- Extra groot lettertype
- Nee
- Product breedte
- 162 mm
- Product hoogte
- 20 mm
- Product lengte
- 242 mm
- Studieboek
- Nee
- Verpakking breedte
- 162 mm
- Verpakking hoogte
- 22 mm
- Verpakking lengte
- 244 mm
- Verpakkingsgewicht
- 570 g
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- EAN
- 9780471322498
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